Crystallization and preliminary X-ray diffraction analysis of the amidase domain of allophanate hydrolase from Pseudomonas sp. strain ADP

نویسندگان

  • Sahil Balotra
  • Janet Newman
  • Nigel G. French
  • Lyndall J. Briggs
  • Thomas S. Peat
  • Colin Scott
چکیده

The allophanate hydrolase from Pseudomonas sp. strain ADP was expressed and purified, and a tryptic digest fragment was subsequently identified, expressed and purified. This 50 kDa construct retained amidase activity and was crystallized. The crystals diffracted to 2.5 Å resolution and adopted space group P21, with unit-cell parameters a = 82.4, b = 179.2, c = 112.6 Å, β = 106.6°.

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عنوان ژورنال:

دوره 70  شماره 

صفحات  -

تاریخ انتشار 2014